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Doxorubicin has been reported to induce significant senescence in breast cancer cell lines, particularly promoting SA--gal expression in MCF7 cells line indicating a substantial impact of p53 on senescence induction [58]

Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off

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