Figure 13 4 Discussion 4.1 Effect of glutathione on growth performance and hepatic lipid metabolism in bullfrogs Relatively many studies have been conducted to show that glutathione has a promoting effect on the growth and antioxidant capacity of aquatic animals ( 4.2 Serum antioxidant capacity and effect on hepatic lipid metabolizing capacity in bullfrogs with glutathione The animal body due to the normal metabolic process is prone to produce many less stable unpaired electron molecules, atomic groups or chemical groups ( Total cholesterol, including free cholesterol and cholesteryl esters, is synthesized and stored in the liver, and its serum concentration can be used as an indicator of lipid metabolism ( 4.3 Regulation of serum metabolites and hepatic metabolites in bullfrogs by glutathione In the serum metabolomics and liver metabolite assay of bullfrogs, it was found that the significant differential metabolites in the three comparisons were mainly lipids and lipid-like molecules, as well as some organic acids and their derivatives
Lipid droplet volume was quantified using the Hybrid Cell Count application (Keyence) and normalized to nuclear signal intensity
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